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KMID : 1123920030170010220
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Korean Journal of Oriental Physiology and Pathology
2003 Volume.17 No. 1 p.220 ~ p.224
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Effects of Sophorae Radix Extract in Rat Cardiac Endothelial Cells
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Kwon Kang-Beom
Park Cheon-Su
Kim In-Gyu
Kim Hyun-Kyu
Choi Ki-Bang
Kim Yong-Bok
Ryu Do-Gon
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Abstract
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To test the protective effect of Sophorae Radix (SR) on the damage of cardiac endothelial cells by xanthine oxidase (XO)/hypoxanthine (HX)-induced oxygen tree radical, Neutral Red (NR), lactate dyhydrogenase (LDH), and c-fos immunopositive cells assay were used in the presence of SR extract. The results of these experiments were obtained as follows ; Cardiac endothelial cells treated with XO/HX showed the cytotoxicity such as a decrease in viability, and increases in LDH activity and c-fos immunopositive cells. Cardiac endothelial cells pretreated with SR extract protected the increase of LDH activity. Alos, cardiac endothelial cells pretreated with SR extract inhibited the increase of c-fos immunopositive cells. These results show that XO/HX induces toxic effects in cultured cardiac endothelial cells derived from neonatal rat, and suggest that SR extract is very effective in the prevention of XO/HX-induced toxicity.
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KEYWORD
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Sophorae Radix (SR), xanthine oxidase/hypoxanthine(XO/HX), cardiac endothelial cells, Neutral Red (NR), lactate dehydrogenase (LDH), c-fos immunopositive cell
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